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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. This type of laboratory testing provides immediate assessment of the patient and can be performed at their bedside:

A) Centralized laboratory
B) High complex testing
C) Molecular testing
D) Point-of-care testing (POCT)

2. The indole (modified Kovacs' reagent) is used to assess the ability of certain bacteria to produce indole by the deamination of tryptophan. Indole reacts with aldehyde in the Kovac's reagent to produce a red color. An alcoholic layer holds the red color as a ring at the top of the tube used for the idole test.
If a bacteria produces the enzyme tryptophanase to break down the amino acid tryptophan, which of the following tests will be positive with Kovac's reagent?

A) esculin test
B) citrate test
C) oxidase test
D) indole test
E) catalase test

3. This patient is most likely suffering from an immediate-acting coagulation inhibitor; most commonly, lupus anticoagulant. Notice that the addition of normal pooled plasma does not correct upon initial or incubated mix, which means that the inhibitor is not time or temperature-dependent.
Factor VIII is not the correct answer as a factor deficiency would have corrected upon the addition of normal pooled plasma. Factor VII is not the correct answer, as the aPTT assay does not account for factor VII activity or concentration.
The laboratorian completed the mixing study ordered for John Doe. The results are as follows:
Initial aPTT result: 167 seconds
Initial 1:1 Mix with Normal Pooled Plasma: 158 seconds
Incubated 1:1 Mix with Normal Pooled Plasma: 150 seconds
Which of the choices below would most likely explain the results for this patient?

A) Factor VII deficiency
B) Time/temperature-dependent coagulation inhibitor
C) Immediate-acting coagulation inhibitor
D) Factor VIII deficiency

4. The cell depicted with the arrow in this image is an atypical (reactive) lymphocyte. These cells are common found in certain viral infections, especially infectious mononucleosis. Notice the larger size and abundant cytoplasm present in this lymphocyte. There is also apparent vacuoliation which is a key feature of atypical lymphocytes. The chromatin pattern of this cell as well as the overall shape, color and size rules out the monocyte, macrophage, and mesothelial cell choices.

A patient with an infectious mononucleosis infection presents in the emergency room. Physicians order a spinal tap which is immediately sent to the laboratory for review. Please identify the cell in the image below from this patient's cerebrospinal fluid sample.

A) Monocyte
B) Mesothelial Cell
C) Reactive Lymphocyte
D) Macrophage

5. The negative antigen frequencies are used in the formula below to determine the percent of type-specific units that would be compatible for the patient. Negative antigen frequencies are determined by subtracting the percent antigen frequency from 100%. The negative antigen frequency for K in this case is .91 (1.00 - .09) and the negative antigen frequency for Fya is .34 (1.00 - .66).
% compatible units available = 100 x (Neg frequency #1 x Neg frequency #2...) For this case, the calculation is:
% compatible units available = 100 x (0.91 x 0.34) = 30.94 or 31%
If the antigen frequencies for K = 0.09 and Fya = 0.66, what percent of type-specific units would be compatible for a patient with anti-K and anti-Fya?

A) 31
B) 43
C) 10
D) 92
E) 100

Solutions:

Question # 1 Answer: D

Question # 2 Answer: D

Question # 3 Answer: C

Question # 4 Answer: C

Question # 5 Answer: A