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American Society of Microbiology ABMM Sample Questions:

1. A patient develops a localized skin infection after being bitten by a tick. The infection is characterized by a slowly expanding erythematous rash with central clearing (erythema migrans). The MOST likely causative agent is:

A) Borrelia burgdorferi
B) Rickettsia rickettsii
C) Anaplasma phagocytophilum
D) Ehrlichia chaffeensis

2. A patient develops a severe diarrheal illness after returning from a backpacking trip where they drank untreated stream water. Stool examination reveals motile trophozoites with multiple flagella and a prominent ventral sucking disk. The MOST likely causative agent is:

A) Cryptosporidium parvum
B) Entamoeba histolytica
C) Giardia lamblia
D) Cyclospora cayetanensis

3. A clinical microbiology laboratory is implementing a new quality control procedure for blood culture media.
To ensure the media supports the growth of a wide range of clinically significant organisms, they should include:

A) A diverse panel of fastidious and non-fastidious bacteria and yeasts.
B) Only Gram-positive and Gram-negative control strains.
C) Only antibiotic-resistant control strains.
D) Only control strains that are commonly found as contaminants.

4. An immunocompromised patient develops a disseminated fungal infection. Blood cultures are negative, but a serum galactomannan assay is strongly positive. A CT scan of the chest reveals multiple nodular lesions with a halo sign. Bronchoalveolar lavage (BAL) fluid is analyzed by PCR, yielding a positive result for Aspergillus fumigatus. The halo sign observed on the CT scan is primarily attributed to:

A) A hypersensitivity reaction in the lung parenchyma.
B) Extensive granuloma formation surrounding fungal hyphae.
C) Angioinvasion by Aspergillus hyphae leading to localized hemorrhage and necrosis.
D) Formation of thick-walled cysts containing fungal conidia.

5. Histopathological examination of lung tissue from an immunocompromised patient reveals large (8-15 µm), spherical yeast cells with thick, refractile walls and characteristic single, broad-based budding. These findings are most suggestive of infection with:

A) Histoplasma capsulatum
B) Coccidioides immitis/posadasii
C) Paracoccidioides brasiliensis
D) Blastomyces dermatitidis

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