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American Society of Microbiology ABMM Sample Questions:

1. Lyme disease, characterized initially by erythema migrans, is caused by Borrelia burgdorferi. What is the primary arthropod vector responsible for transmitting this spirochete to humans in the Northeastern United States?

A) Dermacentor variabilis (American dog tick)
B) Amblyomma americanum (Lone Star tick)
C) Pulex irritans (Human flea)
D) Ixodes scapularis (Blacklegged tick)

2. A clinical microbiology laboratory is implementing a new real-time PCR assay for the direct detection of Chlamydia trachomatis in urine samples. To ensure the accuracy of negative results, it is essential to include:

A) A positive control containing Neisseria gonorrhoeae DNA.
B) An internal amplification control to detect PCR inhibition.
C) A high concentration of the Chlamydia trachomatis target sequence.
D) A melting curve analysis to confirm the specificity of the amplicon.

3. A patient with a history of chronic lung disease develops a severe pneumonia. Bronchoalveolar lavage fluid reveals the presence of numerous cysts containing multiple nuclei, which stain positively with silver stains but poorly with Gram stain. The patient's CD4+ T-cell count is within the normal range. The MOST likely causative organism and the preferred treatment are:

A) Blastomyces dermatitidis; itraconazole.
B) Histoplasma capsulatum; amphotericin B followed by itraconazole.
C) Pneumocystis jirovecii; trimethoprim-sulfamethoxazole.
D) Paragonimus westermani; praziquantel.

4. A patient with a history of travel to Southeast Asia presents with fever, severe headache, and altered mental status. CSF analysis reveals lymphocytic pleocytosis and elevated protein, but routine bacterial and viral cultures are negative. Serological testing of CSF is positive for IgM antibodies against Japanese encephalitis virus. The MOST likely mode of transmission for this infection is:

A) Ingestion of contaminated food or water.
B) Direct contact with infected animals.
C) Respiratory droplets from an infected individual.
D) Bite of an infected mosquito.

5. The human virome, the collection of viruses that reside in and on the human body, is increasinglyrecognized for its role in health and disease. Which of the following statements regarding the human virome is MOST accurate?

A) The human virome is largely independent of the host's immune status.
B) Bacteriophages, viruses that infect bacteria, are a significant component of the human gut virome.
C) The human virome is primarily composed of pathogenic viruses that cause acute infections.
D) The composition of the human virome is relatively stable over time and is not influenced by environmental factors.

Solutions: