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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. A quality control program is to be set up for the following tests:
Phenylalanine deaminase (PAD)
Indole production
Voges-Proskauer (V-P)
Which pair of stock culture organisms would be MOST suitable to verify the performance of these three tests?

A) coli, Enterobacter aerogenes
B) vulgaris, Escherichia coli Klebsiella pneumonia, Proteus vulgaris
C) aerogenes, K. pneumonia Bacillus anthracis, Yersinia pestis

2. Though automated extraction machines have many benefits over manual methods the costs are high and generally require a high throughput of samples in order to justify the costs.
Automated extraction has many benefits over the traditional manual methods. The most important benefit is that the nucleic acid isolated is constantly consistent. There is a reduced amount of manipulation with dramatically decreases the chance of cross contamination. Also, automated extraction machines are considered moderate complexity and can be performed by a wider variety of laboratory professionals.
All of the following are considered benefits of automated isolation and extraction equipment EXCEPT:

A) Automated isolation equipment provides consistent results
B) Automated isolation equipment is cheaper and more practical
C) Automated isolation equipment is considered moderate complexity
D) Automated isolation equipment requires less manipulation

3. The DAT, or direct antiglobulin test, is used to detect antibodies coating RBC's in vivo.
The Direct Antiglobulin Test (DAT) is usually used to detect:

A) Antibodies coating red cells
B) Antibody-hapten reactions
C) Antigens coating red cells
D) Antibodies in the plasma
E) Antigens in the plasma

4. The clue to the identification of the colony seen in the upper frame is the gram stain in the lower frame, in which are seen short, rounded, gram positive bacilli, many of which possess distinct spores. As the colonies grew aerobically, the presence of spores indicates Bacillus species. The colonies are spreading, smooth, yellow-white and non-hemolytic. The lack of hemolysis and the small size of the bacterial cells suggests a species other than Bacillus cereus, the species causing most human infections. The isolate was identified as Bacillus circulans, which is consistent with the gram stain morphology. Clostridium septicum also produces spores; however, this species is an anaerobe and would grow poorly if at all and not produce spores aerobically. Listeria monocytogenes and Lactobacillus species are gram positive bacilli; however, neither of these produce spores.
The colonies seen in the upper frame, grown after 48 hours incubation aerobically at 35°C, and the accompanying gram stain in the lower frame are uncommonly associated with human disease, but have been associated with septicemia, bronchopneumonia, osteomyelitis and other infections, particularly in intravenous drug users. The most likely identification is:

A) Lactobacillus species
B) Listeria monocytogenes
C) Clostridium septicum
D) Bacillus circulans

5. Insulin is the hormone that is mainly responsible for the entry of glucose into the cell for energy production Glucagon and epinephrine promote glycogenolysis, conversion of glycogen to glucose, which increases plasma glucose. Cortisol, along with glucagon, increases gluconeogenesis, formation of glucose from noncarbohydrates, which also raises plasma glucose concentration.
Chemistry
Which of the following hormones is mainly responsible for the entry of glucose into the cell for energy production?

A) Insulin
B) Glucagon
C) Epinephrine
D) Cortisol

Solutions:

Question # 1 Answer: B

Question # 2 Answer: B

Question # 3 Answer: A

Question # 4 Answer: D

Question # 5 Answer: D