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ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Once substance H is developed, the addition of the sugar N-acetylgalactosamine to the terminal position of the chain gives the molecule "A" antigenic activity.
Which specific terminal sugar causes a red cell to have A antigenic activity?

A) Glucose
B) N-acetylgalactosamine
C) Galactose plus glucose
D) Galactose

2. Flow cytometry employs a combination of fluorescent antibody tagging of cells and analysis with laser light scatter.
What principle(s) of flow cytometry are employed when performing immunophenotyping:

A) Defraction gradients
B) Defraction gradients and impedance
C) Fluorescent antibody tagging and light scatter
D) Impedance

3. The normal pH of blood is 7.40. In order for most metabolic processes to take place, the pH must remain within a narrow range close to this value. The range is usually defined in adults as 7.35-7.45. If blood pH falls below 7.35, the blood becomes too acidic (acidosis). If blood rises above 7.45, the blood is too alkaline (alkalosis).
As blood pH decreases, the kidneys will retain bicarbonate (HCO3-) from the glomerular filtrate; therefore, bicarbonate is increased. However, in this case, the increased HCO3- could not compensate for the markedly elevated pCO2 (the respiratory component) and the condition that results is uncompensated respiratory acidosis.
What condition is indicated by the following blood gas results: Bicarbonate = 32 mmol/L (Normal = 22 - 26 mmol/L); pCO2 = 65 mm Hg (Normal = 35 - 45 mmHg); pH = 7.28 (normal = 7.35 - 7.45)

A) Compensated metabolic acidosis
B) Uncompensated respiratory acidosis
C) Healthy condition
D) Uncompensated metabolic acidosis

4. Listeria are non-capsulated, non-acid-fast, ß-hemolytic, gram-positive bacilli that have a characteristic tumbling motility. They are found primarily in the environment and in the human GI tract. Since L.
monocytogenes multiplies intracellularly, control of listeriosis requires cell-mediated immunity; thus, immunocompromised patients (such as newborns) are at high risk.
Which bacterial species is a common agent of neonatal bacteremia?

A) Listeria monocytogenes
B) Staphylococcus aureus
C) Enterococcus faecium
D) Streptococcus pyogenes

5. The tube of choice for trace metal analysis is:

A) red
B) light blue
C) royal blue
D) gold

Solutions: